FastQCFastQC Report
Thu 2 Jun 2016
DRR015367_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015367_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9768103
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211600.21662343241057144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166190.1701353886215164No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144750.051.380081
GTATCAA266700.042.341381
ATCAACG312500.035.5978163
TCAACGC314100.035.430794
CAACGCA330750.033.6609545
TATCAAC341250.032.8918842
AACGCAG343250.032.5411766
ACGCAGA388000.028.2953877
CGCAGAG392250.027.8032238
GTACATG325350.026.6784861
TACATGG319700.025.6877962
GCAGAGT429650.024.7582389
ACATGGG334450.023.3987033
GTGGTAT60500.022.3440741
CAGAGTA404700.021.00599710-11
CATGGGG210700.019.7150784
AGAGTAC389250.019.61312310-11
GAGTACT254900.019.58033812-13
GTACTTT257100.019.06771314-15
AGTACTT249900.018.03315212-13
Produced by FastQC (version 0.11.5)