FastQCFastQC Report
Thu 2 Jun 2016
DRR015367_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015367_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9768103
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212890.21794405730570202No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160130.16393152283508886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98460.10079746292601542No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137700.050.8705441
GTATCAA260250.043.0024221
ATCAACG316900.034.8883
TCAACGC316750.034.8614274
CAACGCA331600.033.3002365
TATCAAC340850.032.4899672
AACGCAG342800.032.2918786
ACGCAGA395700.027.5724357
GTACATG313300.026.9435711
CGCAGAG403350.026.812618
TACATGG311750.025.5985372
GTGGTAT59100.024.5385441
ACATGGG320450.024.1368523
GCAGAGT439100.024.1222749
TGGTATC68750.020.2507322
AGAGTAC399050.020.06707210-11
GAGTACT268250.019.8107912-13
CAGAGTA418150.019.79787610-11
CATGGGG204800.019.4610464
GTACTTT270250.019.19277814-15
Produced by FastQC (version 0.11.5)