FastQCFastQC Report
Thu 2 Jun 2016
DRR015366_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015366_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6746953
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201470.2986088683291554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166620.24695592217701826No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90090.13352694171724627No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118150.056.8617171
GTATCAA220550.041.626141
ATCAACG263050.034.333063
TCAACGC264400.034.1402934
TATCAAC278300.032.5014762
CAACGCA277450.032.4688955
AACGCAG284600.031.845026
ACGCAGA318900.028.1055647
CGCAGAG319150.027.8126938
GTACATG208750.025.794411
GCAGAGT348050.025.0065579
TACATGG208500.024.6806432
GTGGTAT47600.023.8100191
ACATGGG210750.023.4019473
TGGTATC48750.022.1194152
GAGTACT226100.022.05416512-13
CAGAGTA336250.021.80462510-11
AGAGTAC314350.020.83429710-11
TATAACG6350.020.778982
CATGGGT74500.020.4585864
Produced by FastQC (version 0.11.5)