FastQCFastQC Report
Thu 2 Jun 2016
DRR015366_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015366_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6746953
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188200.27894073072689257No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147450.21854309641700484No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90670.13438658902766923No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103550.053.90411
GTATCAA200150.042.492641
TCAACGC245800.034.262784
ATCAACG246000.034.253423
CAACGCA255150.032.9893845
TATCAAC259300.032.584232
AACGCAG264100.031.9403296
ACGCAGA302400.027.594067
CGCAGAG301600.027.4258828
GTACATG206000.026.7196351
TACATGG203850.025.4443992
ACATGGG202950.024.6156523
GCAGAGT332600.024.2267079
GTGGTAT46300.022.3581241
CATGGGT76400.021.6773474
AGAGTAC306650.020.69062410-11
TGGTATC46300.020.5382482
GAGTACT224000.020.2509412-13
CAGAGTA325400.019.8899110-11
AGTACTT231350.019.20440112-13
Produced by FastQC (version 0.11.5)