FastQCFastQC Report
Thu 2 Jun 2016
DRR015365_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015365_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8559042
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249290.29125923204956816No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197950.23127588344583425No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114080.1332859448522393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132600.053.8480951
GTATCAA251650.041.1229671
ATCAACG298600.033.9207423
TCAACGC301000.033.680124
CAACGCA313350.032.338175
TATCAAC322450.031.6663532
AACGCAG324100.031.4480636
GTACATG286300.029.581211
TACATGG287600.028.462952
ACGCAGA370100.027.2688857
ACATGGG288900.027.2161183
CGCAGAG374300.026.8291848
GCAGAGT410200.023.8580069
CATGGGT79950.022.7649364
CATGGGG163050.021.7670084
TACCTGG65850.021.7666872
GTGGTAT58300.021.5442521
CAGAGTA396450.020.36441410-11
GTACCTG77450.019.7428021
GAGTACT268550.019.71176712-13
Produced by FastQC (version 0.11.5)