FastQCFastQC Report
Thu 2 Jun 2016
DRR015365_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015365_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8559042
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269380.31473148513583643No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202300.23635822794186545No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140780.16448102486236194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA135550.053.419761
GTATCAA258000.042.3112071
ATCAACG316200.033.8843843
TCAACGC318800.033.493874
CAACGCA330850.032.3289835
TATCAAC334200.032.29082
AACGCAG339600.031.5763876
GTACATG266700.028.415741
ACGCAGA390650.027.3335367
TACATGG264150.027.2819332
CGCAGAG395000.026.8827888
ACATGGG269550.025.4526233
GCAGAGT426350.024.5252089
TACCTGG69950.021.3332332
CATGGGT83400.021.2749774
AGAGTAC397500.020.46414610-11
GTGGTAT59300.020.3001941
CAGAGTA416150.020.19769110-11
GTACCTG78300.019.9166071
GAGTACT297200.019.81425912-13
Produced by FastQC (version 0.11.5)