FastQCFastQC Report
Thu 2 Jun 2016
DRR015364_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015364_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11671078
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289900.2483917938000243No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225690.19337545340713172No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133430.11432534338301913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA163850.053.886321
GTATCAA303750.040.799481
TCAACGC358250.033.9008984
ATCAACG360100.033.739663
CAACGCA376750.032.2483025
TATCAAC389650.031.4732952
AACGCAG389300.031.3371436
ACGCAGA439300.027.5323057
CGCAGAG441350.027.1774838
GTACATG318900.026.4307631
TACATGG321450.024.9289532
GCAGAGT483750.024.2179899
ACATGGG325050.023.7845943
GTGGTAT74550.020.6344221
CAGAGTA473350.020.57363110-11
GAGTACT326400.020.38561612-13
AGAGTAC445400.020.22010410-11
CATGGGG198650.019.562224
GAGTACA237800.018.775021
TGGTATC73450.018.6480242
Produced by FastQC (version 0.11.5)