FastQCFastQC Report
Thu 2 Jun 2016
DRR015364_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015364_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11671078
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295330.25304432032756524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218110.18688076628397138No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152960.13105901614229637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA167750.051.810351
GTATCAA313400.041.6851841
ATCAACG383600.033.420913
TCAACGC385450.033.225094
CAACGCA398700.032.1095125
TATCAAC408850.031.4236432
AACGCAG411350.031.2105456
ACGCAGA472200.026.9766437
CGCAGAG474250.026.6489898
GTACATG302600.025.64761
TACATGG304600.024.3900182
GCAGAGT522000.023.8311659
ACATGGG305800.023.4909443
GTGGTAT76250.021.2297381
AGAGTAC479500.020.62853210-11
GAGTACT358750.020.17661712-13
CAGAGTA509050.019.66349810-11
AGTACTT372550.019.30096812-13
TGGTATC81450.018.3205532
GTACTTT396250.017.82497814-15
Produced by FastQC (version 0.11.5)