FastQCFastQC Report
Thu 2 Jun 2016
DRR015363_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015363_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14867354
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT348270.23425150164582076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269230.18108804027939335No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156030.10494806271512738No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA208200.053.185481
GTATCAA401850.041.6108741
ATCAACG469850.034.9796143
TCAACGC470300.034.9074444
CAACGCA495550.033.1196065
AACGCAG510300.032.2425466
TATCAAC515000.032.1427762
ACGCAGA573000.028.3570947
CGCAGAG576400.028.0239528
GTACATG432650.027.62421
TACATGG431650.026.2374172
ACATGGG437150.024.9808523
GCAGAGT635500.024.7995369
CAGAGTA625750.020.40898110-11
GTGGTAT92050.020.2717741
AGAGTAC586000.020.21729710-11
CATGGGG254800.019.804264
GAGTACT429900.019.6738912-13
CATGGGT122500.019.537824
GAGTACA316650.018.929451
Produced by FastQC (version 0.11.5)