FastQCFastQC Report
Thu 2 Jun 2016
DRR015363_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015363_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14867354
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT364160.24493934831981537No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261800.1760905134834349No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186440.12540227400248896No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA195600.054.0233881
GTATCAA400900.045.014731
ATCAACG494900.035.7780653
TCAACGC496700.035.666734
CAACGCA516750.034.2388345
AACGCAG532500.033.2944836
TATCAAC535600.033.288652
ACGCAGA604050.029.041947
CGCAGAG607400.028.7169848
GTACATG385900.027.5599481
TACATGG390450.025.878832
GCAGAGT672650.025.4546139
ACATGGG396450.024.5232283
AGAGTAC621800.021.72220610-11
CAGAGTA658400.019.9617310-11
GAGTACT470650.019.79808612-13
AGTACTT492450.019.66558312-13
CATGGGG231950.019.653274
GTGGTAT87600.018.4819971
GTACTTT507950.018.04412714-15
Produced by FastQC (version 0.11.5)