FastQCFastQC Report
Thu 2 Jun 2016
DRR015362_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015362_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12627256
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360570.28554897437733107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293700.23259210076995349No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167910.1329742582236394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA193350.054.2519381
GTATCAA365550.041.904031
ATCAACG438100.034.3058973
TCAACGC439650.034.2263534
CAACGCA460000.032.6627465
TATCAAC472650.031.9814822
AACGCAG475000.031.660036
ACGCAGA534450.027.8232887
CGCAGAG538600.027.4568378
GTACATG369600.026.238561
TACATGG363450.025.2522352
GCAGAGT585400.024.5829249
ACATGGG370250.023.866443
GTGGTAT83700.023.1618181
CAGAGTA564750.020.89780810-11
TGGTATC83750.020.7547342
AGAGTAC541050.020.27434510-11
GAGTACT403300.020.18771212-13
CATGGGG215450.019.4300884
AGTACTT414400.018.65336612-13
Produced by FastQC (version 0.11.5)