FastQCFastQC Report
Thu 2 Jun 2016
DRR015362_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015362_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12627256
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT372140.2947116934985716No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291590.23092111223531067No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198430.1571441966488998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA187350.054.734171
GTATCAA353800.042.5541531
ATCAACG449050.033.0283363
TCAACGC453150.032.7194674
CAACGCA468600.031.6406885
TATCAAC482000.030.9403862
AACGCAG486400.030.5856726
GTACATG335200.026.9465541
ACGCAGA555600.026.5060067
CGCAGAG556350.026.1922428
TACATGG333650.025.0348722
GTGGTAT79800.024.3404961
GCAGAGT602150.023.6817179
ACATGGG338550.023.3824633
TGGTATC86100.021.3472562
AGAGTAC555200.020.89732410-11
CAGAGTA583400.019.81699810-11
GAGTACT426500.019.81461712-13
AGTACTT444800.019.39330512-13
CATGGGT97250.018.1980084
Produced by FastQC (version 0.11.5)