FastQCFastQC Report
Thu 2 Jun 2016
DRR015361_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015361_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11156255
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259190.23232706674417178No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210270.18847722645278364No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114500.10263300722330208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161500.055.17471
GTATCAA308700.041.8384481
ATCAACG372000.034.2362173
TCAACGC373050.034.139864
CAACGCA393000.032.3720665
TATCAAC398800.032.0267682
AACGCAG405100.031.5062316
GTACATG345000.027.7408621
ACGCAGA457400.027.5655217
CGCAGAG462900.027.0904318
TACATGG347600.026.285112
ACATGGG344900.025.5014293
GCAGAGT501000.024.356229
GTGGTAT67350.021.2849561
CAGAGTA485100.020.47900410-11
CATGGGG203550.020.207994
AGAGTAC458400.020.18799810-11
GAGTACT328600.020.08321212-13
CATGGGT99250.019.4842744
TGGTATC70550.018.8971562
Produced by FastQC (version 0.11.5)