FastQCFastQC Report
Thu 2 Jun 2016
DRR015361_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015361_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11156255
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263420.23611866168351298No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205660.18434501541960094No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133990.12010302740480565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA159050.053.485551
GTATCAA311850.043.426591
ATCAACG382850.034.686973
TCAACGC383500.034.4976844
CAACGCA401650.032.9387785
TATCAAC407300.032.7499352
AACGCAG416450.031.8337336
GTACATG326200.027.8126321
ACGCAGA478400.027.4832157
CGCAGAG478400.027.2748
TACATGG326600.026.2855222
ACATGGG331750.024.7118233
GCAGAGT524650.024.5005519
GTGGTAT69700.021.465071
AGAGTAC480400.020.65586910-11
CAGAGTA508600.019.97138610-11
CATGGGG196200.019.8488644
GAGTACT347150.019.84741212-13
TGGTATC73050.019.119612
AGTACTT358200.019.09539412-13
Produced by FastQC (version 0.11.5)