FastQCFastQC Report
Thu 2 Jun 2016
DRR015360_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015360_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8802615
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231700.2632172371505513No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188470.21410683075427017No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104860.11912369222100477No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128700.054.8809931
GTATCAA245450.041.19931
ATCAACG289800.034.151363
TCAACGC292450.033.857464
CAACGCA304900.032.4003375
TATCAAC312550.031.7820072
AACGCAG317450.031.2339236
ACGCAGA361500.027.126027
GTACATG257950.026.7997671
CGCAGAG363250.026.7822288
TACATGG263000.025.035712
GTGGTAT58950.024.0868261
GCAGAGT395800.023.955489
ACATGGG263600.023.9429763
CAGAGTA382500.020.38136110-11
TGGTATC62650.020.1916052
AGAGTAC358800.020.12356810-11
GAGTACT262050.020.02690912-13
GAGTACA190000.019.1142861
CATGGGG155000.019.0822144
Produced by FastQC (version 0.11.5)