FastQCFastQC Report
Thu 2 Jun 2016
DRR015360_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015360_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8802615
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232960.26464862998097727No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186500.21186885942415973No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117570.13356258339141266No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128950.051.488711
GTATCAA249900.042.6993831
ATCAACG306900.034.169293
TCAACGC310650.033.6982354
CAACGCA322750.032.420785
TATCAAC325850.032.3496972
AACGCAG334400.031.372926
ACGCAGA384200.027.1879467
CGCAGAG385650.026.9559578
GTACATG241350.025.7336771
TATAACG7800.025.0804752
GCAGAGT421850.024.2358749
TACATGG241000.024.1820982
ACATGGG242650.022.948963
GTGGTAT61400.022.9489141
AGAGTAC389250.020.49496510-11
TGGTATC63650.020.3708132
CAGAGTA408650.019.65563410-11
GAGTACT292650.019.60972812-13
ATAACGC10750.019.044343
Produced by FastQC (version 0.11.5)