FastQCFastQC Report
Thu 2 Jun 2016
DRR015359_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015359_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9937229
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229300.23074843097607994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191200.19240776276766894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112230.11293892895091781No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140500.054.001151
GTATCAA250100.040.7277221
TCAACGC298650.033.5482254
ATCAACG300800.033.3084343
CAACGCA314750.031.7454385
TATCAAC322200.031.2514842
AACGCAG326150.030.747446
GTACATG291350.028.509631
TACATGG292150.027.4107882
ACGCAGA370350.026.8812687
CGCAGAG373050.026.430448
ACATGGG292950.025.9846693
GCAGAGT407800.023.6076419
GAGTACA209550.021.7632521
CATGGGG190400.021.507524
GTGGTAT58150.020.9762951
CAGAGTA393250.020.54736910-11
GAGTACT281650.019.86896112-13
AGAGTAC374450.019.45875710-11
AGTACAT214850.019.314092
Produced by FastQC (version 0.11.5)