FastQCFastQC Report
Thu 2 Jun 2016
DRR015359_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015359_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9937229
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216090.21745498669699573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173300.1743946929269719No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122580.12335430732249404No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131100.052.397991
GTATCAA247650.041.4047661
ATCAACG301900.033.5754623
TCAACGC303800.033.2905964
CAACGCA313850.032.1231085
AACGCAG325900.031.0191296
TATCAAC328100.030.9775332
GTACATG274450.028.0045781
TACATGG274850.026.401882
ACGCAGA382750.026.0909087
CGCAGAG386400.025.6560638
ACATGGG275750.025.2102833
GCAGAGT424550.022.5710569
CATGGGG182300.020.4393524
AGAGTAC392550.019.8059610-11
CAGAGTA411050.019.06401410-11
GAGTACT294200.018.50010712-13
GTGGTAT59950.018.1084021
AGTACTT305000.018.0539112-13
GAGTACA200450.016.9073311
Produced by FastQC (version 0.11.5)