FastQCFastQC Report
Thu 2 Jun 2016
DRR015358_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015358_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11400678
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293840.25773905727361124No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223100.19569011597380437No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140330.12308917066160452No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA158100.055.040481
GTATCAA312100.043.12051
ATCAACG380250.034.797693
TCAACGC381500.034.659824
CAACGCA403050.032.7727855
TATCAAC412800.032.1974072
AACGCAG417500.031.7696
GTACATG392250.029.7728161
TACATGG389200.028.8069272
ACGCAGA473800.027.6773287
CGCAGAG474400.027.4695578
ACATGGG398750.027.1243
GCAGAGT520300.024.4670169
CATGGGT125100.024.1146774
CATGGGG218150.022.0050564
GAGTACA277100.020.7111761
AGAGTAC477100.020.5601610-11
CAGAGTA508750.020.0099410-11
GAGTACT338150.019.91295212-13
ATAACGC12900.019.047123
Produced by FastQC (version 0.11.5)