FastQCFastQC Report
Thu 2 Jun 2016
DRR015358_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015358_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11400678
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283500.24866942123968416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205100.17990158129191966No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153080.13427271606127286No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA150650.054.7644651
GTATCAA304150.044.4596751
ATCAACG382800.034.4543043
TCAACGC386000.034.0625954
CAACGCA403050.032.6668175
TATCAAC415350.031.8527832
AACGCAG416950.031.643266
GTACATG364750.029.258361
TACATGG359100.028.505872
ACGCAGA481500.027.1932877
CGCAGAG480600.027.0075518
ACATGGG370700.026.7179453
GCAGAGT530350.023.9960089
CATGGGT120050.021.6391964
CATGGGG208200.021.5239874
AGAGTAC485750.021.22839710-11
CAGAGTA513500.019.45199610-11
GAGTACT352950.019.43666812-13
AGTACTT369100.019.36911812-13
GAGTACA255800.018.7383611
Produced by FastQC (version 0.11.5)