FastQCFastQC Report
Thu 2 Jun 2016
DRR015357_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015357_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8309164
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218820.26334779287061855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174860.21044235015700738No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102180.12297266006544101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA165800.041.093241
GTATCAA281700.035.5442121
ATCAACG327900.030.02873
TCAACGC329550.029.878174
CAACGCA340050.028.9555995
TATCAAC346700.028.6105332
GTACATG266950.028.438011
AACGCAG353200.028.0063746
TACATGG266550.027.3297672
ACATGGG269200.026.0464343
ACGCAGA395550.024.6857537
CGCAGAG396600.024.505678
CATGGGT97500.024.03374
GCAGAGT427850.022.0759759
AGAGTAC352950.020.11555710-11
GAGTACT250800.020.0817512-13
GAGTACA193550.019.7758831
CATGGGG137500.019.4577524
TACCTGG59550.018.490852
CAGAGTA416750.018.48258810-11
Produced by FastQC (version 0.11.5)