FastQCFastQC Report
Thu 2 Jun 2016
DRR015357_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015357_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8309164
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234840.2826277108021938No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176490.21240403968437738No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122490.14741555227457298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA167800.039.5740241
GTATCAA286850.036.441861
ATCAACG343450.029.977063
TCAACGC344200.029.8456544
CAACGCA356600.028.7823185
TATCAAC359950.028.7166752
AACGCAG368200.027.9867486
GTACATG250700.027.3247641
TACATGG251850.025.9586932
ACATGGG257200.024.6227343
ACGCAGA421550.024.2074017
CGCAGAG425550.023.8515728
CATGGGT95300.022.4374224
GCAGAGT458900.021.6844489
AGAGTAC384800.020.15764210-11
GAGTACT277550.019.9949812-13
AGTACTT293850.018.97876712-13
CATGGGG131900.018.1429944
GTACTTT307650.017.92036614-15
CAGAGTA451950.017.61072310-11
Produced by FastQC (version 0.11.5)