FastQCFastQC Report
Thu 2 Jun 2016
DRR015356_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015356_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8071323
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224400.27802133553569847No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186240.23074284104353154No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115300.14285142596820868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124600.054.8618241
GTATCAA233650.040.5539631
ATCAACG279500.033.4068763
TCAACGC280550.033.2656254
CAACGCA292300.031.8038645
TATCAAC298000.031.3789622
AACGCAG300650.031.0870516
GTACATG243900.028.4188461
TACATGG243600.027.27212
ACGCAGA343250.026.910757
CGCAGAG346750.026.5470988
ACATGGG247150.025.849183
GCAGAGT373800.023.9047019
CATGGGT79850.023.1931534
GTGGTAT55150.022.8587571
CAGAGTA366750.020.53081910-11
GAGTACT261350.020.2108912-13
AGAGTAC349000.020.01717610-11
CATGGGG138500.019.9424824
TGGTATC57700.019.636612
Produced by FastQC (version 0.11.5)