FastQCFastQC Report
Thu 2 Jun 2016
DRR015356_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015356_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8071323
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225790.2797434819545693No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179840.2228135337911765No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126060.1561825737862306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117800.053.861761
GTATCAA224400.042.2397541
ATCAACG277750.033.578533
TCAACGC279550.033.2646754
CAACGCA289100.032.1972965
TATCAAC294700.031.7398472
AACGCAG297450.031.369936
GTACATG221650.028.0701871
ACGCAGA339600.027.2754547
CGCAGAG343800.026.7437528
TACATGG225100.026.3752692
ACATGGG226600.025.2972033
GCAGAGT376150.024.0353139
GTGGTAT55600.021.3303151
AGAGTAC350800.020.7367210-11
CAGAGTA369200.020.00524310-11
CATGGGT75800.019.8664674
GAGTACT273200.019.6976412-13
CATGGGG128750.019.6466924
TGGTATC58750.018.6625522
Produced by FastQC (version 0.11.5)