FastQCFastQC Report
Thu 2 Jun 2016
DRR015355_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015355_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9312074
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227020.24379101798374883No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185660.19937556338147658No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103050.11066278038598062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131000.053.156541
GTATCAA247300.040.636051
ATCAACG290250.034.3321653
TCAACGC290750.034.3197024
CAACGCA306450.032.6059845
TATCAAC312200.031.962252
AACGCAG315800.031.6836626
ACGCAGA357400.027.7413317
CGCAGAG360750.027.2692998
GTACATG261800.027.0158061
TACATGG264000.025.3191722
GCAGAGT394750.024.307589
ACATGGG265200.024.0549133
GTGGTAT62550.020.8101541
CAGAGTA383150.020.51301810-11
GAGTACT264750.020.3552812-13
CATGGGG172800.020.3018324
AGAGTAC359450.020.11248410-11
GAGTACA192450.019.2505471
AGTACTT275600.018.44787412-13
Produced by FastQC (version 0.11.5)