FastQCFastQC Report
Thu 2 Jun 2016
DRR015355_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015355_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9312074
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228940.2458528572689607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175560.18852942964155997No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119260.12807028810123286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125200.053.0467261
GTATCAA247450.043.1718831
ATCAACG304250.034.436643
TCAACGC304950.034.357594
CAACGCA318300.032.9308745
TATCAAC324300.032.3496632
AACGCAG328900.031.9110536
ACGCAGA384300.027.0858847
CGCAGAG383300.026.9547758
GTACATG251050.025.9822861
TACATGG249650.024.5284962
GCAGAGT421200.024.2089659
ACATGGG252100.023.2614863
GTGGTAT62100.020.7137641
AGAGTAC387400.020.69285410-11
GAGTACT285200.019.59556812-13
CAGAGTA414350.019.31949810-11
AGTACTT298200.018.89391712-13
TGGTATC62150.018.8126262
CATGGGG164300.018.4414084
Produced by FastQC (version 0.11.5)