FastQCFastQC Report
Thu 2 Jun 2016
DRR015354_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015354_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10676557
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199020.18640840862836214No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162020.1517530417343344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144200.051.3164521
GTATCAA264900.040.7506031
TCAACGC311050.034.12834
ATCAACG311600.034.0974273
CAACGCA328150.032.3221325
AACGCAG338850.031.3547576
TATCAAC340000.031.3431172
ACGCAGA385150.027.243297
CGCAGAG385850.026.8635438
GTACATG285750.026.6606371
TACATGG284150.025.541552
ACATGGG290300.024.0442093
GCAGAGT421050.023.9561719
GAGTACT268950.020.48603612-13
CAGAGTA412000.020.04861610-11
AGAGTAC379600.019.84213310-11
CATGGGG189800.019.4665934
GAGTACA212250.018.954221
GTACTTT293950.018.44939214-15
AGTACTT281650.018.01960412-13
Produced by FastQC (version 0.11.5)