FastQCFastQC Report
Thu 2 Jun 2016
DRR015354_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015354_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10676557
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201650.18887174957245112No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155770.14589909462385675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137050.050.44571
GTATCAA262150.042.2727431
ATCAACG316150.034.248793
TCAACGC317550.034.040494
CAACGCA329650.032.777215
AACGCAG340800.031.758236
TATCAAC346200.031.4468442
ACGCAGA395000.026.8937427
CGCAGAG397650.026.5657848
GTACATG262900.025.7769931
GCAGAGT436300.023.819659
TACATGG266400.023.7720412
ACATGGG265850.022.8800093
AGAGTAC392250.020.56396110-11
GAGTACT287800.019.72601512-13
CAGAGTA424150.019.24803710-11
AGTACTT301350.019.05046812-13
CATGGGG177600.018.315744
GTACTTT313050.017.8514814-15
GTGGTAT69600.017.6912521
Produced by FastQC (version 0.11.5)