FastQCFastQC Report
Thu 2 Jun 2016
DRR015353_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015353_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11491405
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199970.17401701532580222No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170830.14865893248040601No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA162800.053.8147541
GTATCAA295750.039.9950751
ATCAACG346850.033.3459853
TCAACGC347800.033.3203124
CAACGCA360800.032.0566945
AACGCAG373300.031.080786
TATCAAC374350.031.0426242
GTACATG343000.026.710051
ACGCAGA432550.026.4656247
CGCAGAG436700.026.0362038
TACATGG339800.025.3345472
ACATGGG342350.023.9493733
GCAGAGT478000.023.1562989
GTGGTAT69850.022.2835661
CAGAGTA458450.020.29346710-11
GAGTACT294550.019.50739312-13
AGAGTAC430700.018.80182810-11
CATGGGG207300.018.7442594
GTACTTT304850.018.57221214-15
TGGTATC76000.018.5593812
Produced by FastQC (version 0.11.5)