FastQCFastQC Report
Thu 2 Jun 2016
DRR015353_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015353_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11491405
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198060.17235490351266883No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163140.14196697444742395No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA153450.050.5460321
GTATCAA291850.042.0061651
ATCAACG359500.033.494623
TCAACGC360750.033.3029024
CAACGCA373300.032.122365
TATCAAC384700.031.4656752
AACGCAG387400.031.0001876
ACGCAGA449150.026.3736137
CGCAGAG454300.025.8142918
GTACATG320400.025.290391
TACATGG314750.024.092632
GCAGAGT489150.023.3675359
ACATGGG325000.022.4512773
GTGGTAT71600.021.92051
AGAGTAC451350.019.77755510-11
CAGAGTA480650.018.85124410-11
AGTACTT320450.018.36985612-13
CCGGTCG17150.018.3071339
GAGTACT320650.018.26614412-13
TGGTATC81400.018.1624032
Produced by FastQC (version 0.11.5)