FastQCFastQC Report
Thu 2 Jun 2016
DRR015352_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015352_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10849932
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT462830.4265741020312385No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT383360.353329403354786No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214970.19813027399618724No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA226450.058.585771
GTATCAA435950.041.7902341
ATCAACG532750.033.9058653
TCAACGC536600.033.721954
CAACGCA557350.032.490985
TATCAAC559700.032.2735672
AACGCAG575500.031.6718466
GTGGTAT81650.028.1488781
GTACATG443550.027.8479461
ACGCAGA649850.027.7751797
CGCAGAG650950.027.5528628
TACATGG437800.027.073462
TGGTATC85650.026.2428722
ACATGGG444100.025.7055953
GCAGAGT689600.025.4124629
CATGGGT152550.024.0994384
CAGAGTA676650.021.6663910-11
GAGTACT459500.021.49968112-13
CATGGGG218500.020.6570364
AGAGTAC646800.020.39791110-11
Produced by FastQC (version 0.11.5)