FastQCFastQC Report
Thu 2 Jun 2016
DRR015352_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015352_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10849932
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT468480.4317815079394046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT368430.3395689484505525No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254020.23412128297209603No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA219850.058.5537641
GTATCAA426950.043.0151061
ATCAACG560700.032.3232153
TCAACGC568850.031.8841064
CAACGCA582800.031.128735
TATCAAC585150.031.1278152
AACGCAG603650.030.121376
GTGGTAT78300.029.1294671
GTACATG419050.026.4632641
TGGTATC84600.025.9715922
ACGCAGA693800.025.9649017
CGCAGAG695700.025.7109018
TACATGG411550.025.5224932
ACATGGG420350.024.3066143
GCAGAGT727200.024.0088759
CATGGGT148800.023.5098154
TATAACG9000.022.7453082
AGAGTAC686350.020.96232810-11
GAGTACT495100.020.5298112-13
CAGAGTA714550.020.30710-11
Produced by FastQC (version 0.11.5)