FastQCFastQC Report
Thu 2 Jun 2016
DRR015351_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015351_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11800420
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT367350.31130247906430447No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304940.2584145310082183No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182560.15470635790929477No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA187900.055.1972051
GTATCAA344650.040.0491831
ATCAACG408900.033.2260253
TCAACGC412400.032.9659654
CAACGCA431950.031.505535
TATCAAC438900.031.1519032
AACGCAG443600.030.6986356
GTACATG365850.028.0506571
TACATGG365000.026.4145852
ACGCAGA511150.026.3568737
CGCAGAG519650.025.767718
ACATGGG370250.024.8971753
GTGGTAT75250.024.6747761
GCAGAGT557400.023.4087059
CATGGGG239500.021.2772924
TGGTATC81850.021.234912
CAGAGTA533950.020.92206610-11
TATAACG12000.020.8538932
GAGTACA263300.020.3953291
GAGTACT384350.019.98070312-13
Produced by FastQC (version 0.11.5)