FastQCFastQC Report
Thu 2 Jun 2016
DRR015351_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015351_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11800420
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT362480.3071755073124516No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277940.23553398946817147No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215440.18256977294028517No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA166400.053.070811
GTATCAA321550.042.6000821
ATCAACG416350.032.3967553
TCAACGC419150.032.1586344
CAACGCA437350.030.8515825
TATCAAC440700.030.6375772
AACGCAG452600.029.882426
GTACATG345450.028.0857451
TACATGG342750.026.9433842
ACATGGG351650.025.4465663
ACGCAGA531100.025.2514677
CGCAGAG537900.024.847678
GTGGTAT70150.023.213691
GCAGAGT578450.022.5209479
AGAGTAC533650.020.67561510-11
CATGGGG226100.020.1806814
TGGTATC77200.019.799562
AGTACTT408250.019.418512-13
CAGAGTA554650.018.90000710-11
GAGTACT407600.018.39437312-13
Produced by FastQC (version 0.11.5)