FastQCFastQC Report
Thu 2 Jun 2016
DRR015350_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015350_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11506470
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267570.23253873690193433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209170.18178468287841537No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127190.11053781046663311No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA175650.048.839891
GTATCAA315050.039.623951
ATCAACG370400.033.4368063
TCAACGC372250.033.160484
CAACGCA392400.031.480865
TATCAAC398050.031.1943132
AACGCAG403000.030.7657266
GTACATG339000.027.252591
ACGCAGA458600.026.5892737
CGCAGAG465950.025.91548
TACATGG345150.025.7588352
ACATGGG355950.024.0951673
GCAGAGT505700.023.1745599
CATGGGG227700.020.1620124
CAGAGTA482100.019.88790310-11
AGAGTAC447550.019.70020510-11
GAGTACT317950.019.6744412-13
GTACTTT327150.018.75256514-15
AGTACTT316800.018.37442212-13
GAGTACA249500.018.0217861
Produced by FastQC (version 0.11.5)