FastQCFastQC Report
Thu 2 Jun 2016
DRR015350_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015350_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11506470
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252650.21957211899044624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191920.1667931172635917No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134320.11673432425409357No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA167700.046.765741
GTATCAA303100.039.436681
ATCAACG362700.032.3207783
TCAACGC366000.032.029364
CAACGCA381050.030.776265
TATCAAC385500.030.5862032
AACGCAG391950.029.990026
GTACATG313850.025.904221
ACGCAGA449850.025.86717
CGCAGAG456100.025.3131688
TACATGG319600.024.0971182
ACATGGG327350.022.6928373
GCAGAGT497050.022.6756529
AGAGTAC441000.020.08624310-11
CAGAGTA471350.019.02461410-11
GAGTACT321700.018.58784912-13
AGTACTT320600.018.42451312-13
CATGGGG215200.017.7985554
GTACTTT331750.017.64066114-15
GAGTACA234650.016.5849231
Produced by FastQC (version 0.11.5)