FastQCFastQC Report
Thu 2 Jun 2016
DRR015349_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015349_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10657683
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276520.2594560187237695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213970.20076596385912399No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129360.12137722617570816No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA243150.035.9548651
GTATCAA382800.033.1931231
TCAACGC450050.027.7316634
ATCAACG450150.027.7153933
GTACATG347250.027.5221041
CAACGCA470700.026.5247175
TATCAAC478350.026.20542
TACATGG341950.026.0534362
AACGCAG484650.025.8738986
ACATGGG349500.024.6441763
ACGCAGA529350.023.2935247
CGCAGAG536200.022.834728
GCAGAGT572250.020.916989
CATGGGG217700.020.7539944
AGAGTAC447150.019.8961310-11
GAGTACT311050.019.83313812-13
GTACTTT316950.019.24854714-15
AGTACTT310250.018.45454812-13
GAGTACA258250.018.4329991
CAGAGTA548900.017.7496110-11
Produced by FastQC (version 0.11.5)