FastQCFastQC Report
Thu 2 Jun 2016
DRR015349_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015349_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10657683
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269110.25250328800359323No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201700.18925314254514794No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145140.13618344625187295No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA240100.033.6707231
GTATCAA383400.033.6279871
ATCAACG460350.027.519893
TCAACGC463050.027.4281924
CAACGCA475550.026.6785355
GTACATG313350.026.1637041
TATCAAC489850.026.1036512
AACGCAG487500.026.0712246
TACATGG313700.024.4771842
ACATGGG317900.023.1238693
ACGCAGA549700.022.815017
CGCAGAG552600.022.5471068
GCAGAGT586250.020.761989
AGAGTAC456600.020.6912910-11
AGTACTT330000.019.42406312-13
GAGTACT332500.019.35329212-13
GTACTTT337300.018.79463614-15
CATGGGG197350.018.3248184
CAGAGTA565700.017.07085810-11
CCGGTCG19500.016.5672939
Produced by FastQC (version 0.11.5)