FastQCFastQC Report
Thu 2 Jun 2016
DRR015348_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015348_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15352263
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT436920.28459647935942733No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT356840.23243478827844466No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206940.13479445994378808No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA241900.052.134051
GTATCAA439400.040.9023131
ATCAACG524850.033.765033
TCAACGC526650.033.6496284
CAACGCA548400.032.2569775
TATCAAC556350.031.9925212
AACGCAG565500.031.3700736
ACGCAGA638250.027.4949997
CGCAGAG642750.027.1890648
GTACATG419800.026.3333381
TACATGG422750.024.8183782
GCAGAGT703700.024.1274939
ACATGGG425950.023.9481453
GTGGTAT105800.020.9490381
GAGTACT474050.020.88779612-13
CAGAGTA677200.020.79898510-11
AGAGTAC636600.020.21735810-11
CATGGGG276450.020.1611064
GTACTTT510400.019.15055714-15
AGTACTT488450.018.8842112-13
Produced by FastQC (version 0.11.5)