FastQCFastQC Report
Thu 2 Jun 2016
DRR015348_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015348_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15352263
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT438350.2855279381287306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328800.21417037996287583No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241620.15738396352381404No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA228400.050.7690431
GTATCAA434100.042.8168831
TCAACGC549350.033.197984
ATCAACG549650.033.196413
CAACGCA571400.031.869125
TATCAAC580100.031.5244792
AACGCAG587400.031.039776
ACGCAGA675150.026.7629327
CGCAGAG680900.026.3565438
GTACATG405000.025.6227551
TACATGG410450.023.9729232
GCAGAGT739650.023.6965549
ACATGGG414050.022.9185053
AGAGTAC673600.021.1864710-11
GTGGTAT106050.020.6117691
AGTACTT532550.019.58147812-13
GAGTACT512250.019.44243212-13
CAGAGTA716500.019.24469610-11
CATGGGG263000.018.1790714
GTACTTT545700.018.07136514-15
Produced by FastQC (version 0.11.5)