FastQCFastQC Report
Thu 2 Jun 2016
DRR015347_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015347_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11482930
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350870.3055579020337144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307310.2676233330691731No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166370.14488462439464492No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA200600.056.8056871
GTATCAA377450.040.968721
ATCAACG457150.033.4412083
TCAACGC460050.033.2798584
CAACGCA482050.031.73275
TATCAAC484950.031.6742922
AACGCAG495300.030.9756666
ACGCAGA554050.027.4857867
CGCAGAG556050.027.3130448
GTACATG351900.026.3038981
TACATGG350750.024.9838892
GCAGAGT596500.024.772039
ACATGGG350700.023.9365773
GTGGTAT83450.023.3948021
TGGTATC83650.022.0287842
GAGTACT405700.021.6424512-13
CAGAGTA589000.021.1907210-11
AGAGTAC553850.020.37936410-11
GTACTTT434700.019.90035614-15
CATGGGG181250.018.9275674
Produced by FastQC (version 0.11.5)