FastQCFastQC Report
Thu 2 Jun 2016
DRR015347_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015347_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11482930
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT333700.29060527234773703No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275100.2395730009675231No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190630.16601163640290414No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA188900.055.529451
GTATCAA358000.040.808881
ATCAACG458550.031.4877153
TCAACGC462950.031.2277534
CAACGCA479200.030.1972775
TATCAAC484750.029.9262262
AACGCAG492800.029.373146
ACGCAGA562000.025.4892647
GTACATG328750.025.4397621
CGCAGAG562450.025.3151998
TACATGG328050.024.1975062
GCAGAGT604650.023.0943879
ACATGGG330200.022.8830283
GTGGTAT79800.021.8412171
AGAGTAC555600.020.77108810-11
TGGTATC82150.020.5992132
CATGGGT113500.020.2402764
GAGTACT413850.019.97398412-13
CAGAGTA591650.019.60546710-11
AGTACTT430700.019.19255412-13
Produced by FastQC (version 0.11.5)