FastQCFastQC Report
Thu 2 Jun 2016
DRR015346_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015346_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13943610
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT408430.292915536220534No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338740.24293565296218123No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185040.13270594917671966No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA231700.056.626791
GTATCAA444250.041.5809971
ATCAACG525650.034.8133543
TCAACGC527950.034.6530724
CAACGCA556100.032.882565
TATCAAC563050.032.5736432
AACGCAG577250.031.7487036
ACGCAGA644750.028.0508677
CGCAGAG645700.027.8891058
GTACATG451700.026.6186491
TACATGG448350.025.6543122
GCAGAGT691850.025.336899
ACATGGG464300.024.1164533
GTGGTAT97300.023.3862441
CAGAGTA679600.021.43887510-11
GAGTACT456500.021.3323712-13
TGGTATC99000.021.2787592
CATGGGT134300.020.3271144
AGAGTAC642600.019.88028310-11
GTACTTT495800.019.40279614-15
Produced by FastQC (version 0.11.5)