FastQCFastQC Report
Thu 2 Jun 2016
DRR015346_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015346_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13943610
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT414740.29744090662317724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314430.2255011435345653No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221760.15904059278766403No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA223100.054.6044121
GTATCAA445850.043.359651
ATCAACG555100.034.3463363
TCAACGC561000.034.001344
CAACGCA580500.032.8513375
TATCAAC590750.032.396272
AACGCAG605950.031.5466526
ACGCAGA696400.027.1096157
CGCAGAG691950.027.0538548
GTACATG440050.025.8589271
GCAGAGT742400.024.6876079
TACATGG439150.024.5091422
GTGGTAT96850.023.1782971
ACATGGG449500.023.0849863
TGGTATC98450.021.4401842
AGAGTAC687000.021.0086310-11
GAGTACT499550.020.29224412-13
CAGAGTA726050.020.01972810-11
AGTACTT513800.019.83130612-13
GTACTTT532000.018.87057914-15
Produced by FastQC (version 0.11.5)