FastQCFastQC Report
Thu 2 Jun 2016
DRR015345_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015345_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12690318
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT356780.28114346701162257No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293080.23094771935581127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161820.12751453509675645No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA200400.055.139041
GTATCAA377150.041.1238441
TCAACGC449850.033.9631734
ATCAACG449500.033.959253
CAACGCA466200.032.733025
TATCAAC473500.032.3341982
AACGCAG482450.031.7813916
ACGCAGA548050.027.719897
CGCAGAG550550.027.4118848
GTACATG365750.025.0725781
GCAGAGT597100.024.3820849
TACATGG365500.023.7264392
GTGGTAT84500.023.536021
ACATGGG371150.022.3231663
TGGTATC87000.021.4418182
GAGTACT401450.020.98830812-13
CAGAGTA580250.020.86008310-11
AGAGTAC549550.019.81500210-11
GTACTTT436950.019.14247514-15
AGTACTT417300.018.3050212-13
Produced by FastQC (version 0.11.5)