FastQCFastQC Report
Thu 2 Jun 2016
DRR015345_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015345_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12690318
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT348700.2747764082822826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268520.21159438242603534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194040.15290396978231752No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA189800.052.5919041
GTATCAA359150.041.982021
ATCAACG451000.033.167023
TCAACGC453150.032.91934
CAACGCA469400.031.7506035
TATCAAC475400.031.5124382
AACGCAG486850.030.6219226
ACGCAGA564450.026.1621997
CGCAGAG567700.025.8120788
GTACATG331850.025.4330081
TACATGG331800.023.9670452
GCAGAGT609700.023.4859819
GTGGTAT85150.023.038361
ACATGGG339500.022.6329443
AGAGTAC565050.020.48791310-11
GAGTACT420950.019.9233512-13
TGGTATC91200.019.903412
AGTACTT437850.019.51291512-13
CAGAGTA590500.019.50857510-11
GTACTTT460300.018.00766814-15
Produced by FastQC (version 0.11.5)