FastQCFastQC Report
Thu 2 Jun 2016
DRR015344_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015344_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11644292
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT299980.25761978486970266No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255440.21936928410933015No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144810.12436136091399976No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA172350.056.875441
GTATCAA324600.040.2368281
ATCAACG382800.033.6009063
TCAACGC384800.033.4262664
CAACGCA406200.031.620455
TATCAAC412500.031.3142662
AACGCAG419000.030.654486
GTACATG353850.030.2488231
TACATGG352550.028.5602172
ACATGGG355550.027.2824653
ACGCAGA469100.027.0702027
CGCAGAG473950.026.6680478
GCAGAGT516000.024.1407629
CATGGGT119550.023.5960454
GTGGTAT73050.022.8631571
CATGGGG200900.021.9226234
CAGAGTA499700.021.1088710-11
GAGTACT352650.020.56410612-13
GAGTACA250450.019.951321
AGAGTAC471800.019.91266410-11
Produced by FastQC (version 0.11.5)