FastQCFastQC Report
Thu 2 Jun 2016
DRR015344_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015344_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11644292
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292710.2513763825228704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229060.19671440736800488No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173680.1491546244288618No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA162800.054.6913531
GTATCAA314250.042.2822841
ATCAACG394450.033.1341173
TCAACGC396400.033.005554
CAACGCA413450.031.655465
TATCAAC422250.031.146032
AACGCAG427750.030.6078266
GTACATG321300.029.4152321
TACATGG324700.027.6699962
ACGCAGA491400.026.3840487
ACATGGG329300.026.1925813
CGCAGAG495200.025.9702978
CATGGGT112600.023.594254
GCAGAGT535500.023.4259229
AGAGTAC497800.020.57335310-11
GTGGTAT74650.019.9807221
GAGTACT381900.019.65547812-13
CATGGGG191800.019.6150154
CAGAGTA526650.019.52410910-11
AGTACTT395700.019.10812-13
Produced by FastQC (version 0.11.5)