FastQCFastQC Report
Thu 2 Jun 2016
DRR015343_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015343_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11529024
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT336920.29223635929632896No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273620.23733145147412307No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151600.13149421841779496No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA185550.055.162591
GTATCAA359950.042.204651
ATCAACG440950.034.0008623
TCAACGC443650.033.814454
CAACGCA464450.032.30015
TATCAAC470900.031.9737642
AACGCAG481700.031.2473146
ACGCAGA542150.027.4197
GTACATG388800.027.2386951
CGCAGAG544000.027.2161968
TACATGG385600.026.3616052
ACATGGG387350.025.3613833
CATGGGT130950.024.9136584
GCAGAGT584450.024.722219
GTGGTAT81500.023.2859841
TGGTATC83500.020.8162562
GAGTACT375900.020.719812-13
CAGAGTA572350.020.65849710-11
AGAGTAC540050.020.63467410-11
CATGGGG203250.019.5437324
Produced by FastQC (version 0.11.5)