FastQCFastQC Report
Thu 2 Jun 2016
DRR015343_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015343_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11529024
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT332910.2887581810914783No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238870.20719013161912056No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183520.15918086387885047No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA172200.052.616821
GTATCAA345200.044.234731
ATCAACG444700.033.8190773
TCAACGC447600.033.630454
CAACGCA464400.032.4334375
TATCAAC471900.032.062292
AACGCAG483350.031.180696
GTACATG360800.026.8575551
ACGCAGA557950.026.6855877
CGCAGAG562600.026.3194798
TACATGG353000.025.9026152
ACATGGG361350.024.3097363
GCAGAGT604400.023.95539
CATGGGT124350.021.986434
AGAGTAC559400.021.02881810-11
GTGGTAT81800.020.9707031
TGGTATC80700.019.7295322
AGTACTT421450.019.69497912-13
GAGTACT406950.019.32880812-13
CAGAGTA590400.019.18471110-11
Produced by FastQC (version 0.11.5)